Anti-MEK1 + MEK2 (phospho S218 + S222) antibody (ab254077)
Key features and details
- Rabbit polyclonal to MEK1 + MEK2 (phospho S218 + S222)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-MEK1 + MEK2 (phospho S218 + S222) antibody
See all MEK1 + MEK2 primary antibodies -
Description
Rabbit polyclonal to MEK1 + MEK2 (phospho S218 + S222) -
Host species
Rabbit -
Specificity
Specific for endogenous levels of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218/222.
Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
-
Immunogen
Synthetic peptide within Human MEK1 (phospho S218 + S222). The exact sequence is proprietary.
Database link: Q02750 -
Positive control
- WB: NIH/3T3 cell lysate (serum treated).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: HEPES, 0.87% Sodium chloride, 50% Glycerol, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MEK1 (phospho S218 + S222) antibody (ab254077)All lanes : Anti-MEK1 + MEK2 (phospho S218 + S222) antibody (ab254077) at 1/1000 dilution
Lane 1 : Serum-starved NIh/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : Serum-treated NIH/3T3 cell lysate
Predicted band size: 43 kDa