Anti-Collagen I antibody (ab34710)
Key features and details
- Rabbit polyclonal to Collagen I
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Collagen I antibody
See all Collagen I primary antibodies -
Description
Rabbit polyclonal to Collagen I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Full length native protein (purified) corresponding to Human Collagen I aa 1-1464. Collagen Type I from human and bovine placenta.
Database link: P02452 -
Positive control
- WB: human collagen. IHC-P: marmoset kidney, normal human placenta, cow small intestine, human colon, human lung.
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General notes
Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.
This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen protein during your experiment.
PLEASE READ THESE IMPORTANT PROTOCOL TIPS, click here for the english version or here for the mandarin version.
It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.8766% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab34710 has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody (ab34710)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labeling Collagen I with ab34710 at 1/400 dilution followed by the addition of Peroxidase goat anti-rabbit secondary antibody at 1/10,000 dilution for 45 min at RT. Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody (ab34710)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal kidney tissue (distal tubules) labeling Collagen I with ab34710 at 1/100 dilution. Samples were incubated with primary antibody (ab34710) for 4 hours at room temperature followed by the addition of secondary antibody and substrate reaction. No antigen retrieval was performed.
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Western blot - Anti-Collagen I antibody (ab34710)Anti-Collagen I antibody (ab34710) at 1/1000 dilution + Human collagen, 50 ng
Secondary
DyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT.SDS-PAGE, DTT added to samples.
DyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT.
Blocking Buffer for 30 min at room temperature - proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
Other Band(s): Collagen Type I splice variants and isoforms.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody (ab34710)IHC image of Collagen I staining in normal human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab34710 at 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody (ab34710) This image is courtesy of an abreview submitted by David KrullImmunohistochemical analysis of human colon tissue sections labelling Collagen I with ab34710 at a dilution of 1/200 for 32 minutes at 35°C. The sections were fixed with formaldehyde. The secondary antibody used was an HRP conjugated rabbit IgG. Antigen retrieval was heat mediated using CC1.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody (ab34710)Immunohistochemistry of breast carcinoma staining Collagen I with ab34710 at 5μg/ml
