Anti-Prothrombin fragment 2 antibody (ab233297)
Key features and details
- Rabbit polyclonal to Prothrombin fragment 2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Prothrombin fragment 2 antibody -
Description
Rabbit polyclonal to Prothrombin fragment 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Orangutan
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Immunogen
Recombinant fragment corresponding to Human Prothrombin fragment 2 aa 1-350. Expressed in E. coli. N-terminal His-tag and GST-tag.
Database link: P00734 -
Positive control
- WB: Recombinant human Prothrombin fragment 2 protein. HEK-293T cell lysate. Pig kidney tissue lysate. Rat serum. IHC-P: Human prostate cancer, pancreatic cancer, colorectal cancer, bile duct cancer and skin cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Prothrombin fragment 2 antibody (ab233297)All lanes : Anti-Prothrombin fragment 2 antibody (ab233297) at 2 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Pig kidney tissue lysate
Lane 3 : Rat serum
Secondary
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)Formalin-fixed, paraffin-embedded human skin cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-Prothrombin fragment 2 antibody (ab233297)Anti-Prothrombin fragment 2 antibody (ab233297) at 2 µg/ml + Recombinant human Prothrombin fragment 2 protein