Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PC1/3 antibody [EPR21908] (ab220363)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21908] to PC1/3
Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-PC1/3 antibody [EPR21908]
See all PC1/3 primary antibodies
Description

Rabbit monoclonal [EPR21908] to PC1/3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Beta-TC-6 whole cell lysate. Mouse hypothalamus lysate. IHC-P: Human pancreas and colon tissue. Mouse and rat pancreas tissue. IHC-Fr: Mouse and rat pancreas tissue. ICC/IF: Beta-TC-6 cells. Flow Cytometry: Beta-TC-6 cells. IP: Beta-TC-6 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Isotype

IgG
Research areas

Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363)

All lanes : Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution

Lane 1 : Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 84 kDa

Exposure time: 125 seconds

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

The PC1/3 protein undergoes multiple intracellular cleavage steps to its 66 kDa mature form; the 87 kDa represents the ProPC1/3 intermediate (PMID: 26778167).

Negative control: NIH/3T3 (PMID: 9405066; PMID:15143067).
Immunohistochemistry (Frozen sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)

Immunohistochemical analysis of frozen rat pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in rat pancreatic islet (PMID: 25976560) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (10mM Tris base pH 9.0, 1mM EDTA, 0.05% Tween 20).
Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363)

Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution + Mouse hypothalamus lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 84 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

The 66 kDa band is the mature form of PC1/3 (PMID: 26778167).
Immunohistochemistry (Frozen sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)

Immunohistochemical analysis of frozen mouse pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in mouse pancreatic islet (PMID: 25976560) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (10mM Tris base pH 9.0, 1mM EDTA, 0.05% Tween 20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in rat pancreatic islets (PMID: 7925129; PMID: 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse pancreatic islets (PMID: 7925129; PMID: 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Sporadic cytoplasmic staining (arrows) in human colon (PMID: 18706454) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human pancreatic islets (PMID: 7925129; PMID: 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunocytochemistry/ Immunofluorescence - Anti-PC1/3 antibody [EPR21908] (ab220363)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labeling PC1/3 with ab220363 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining in Beta-TC-6 cell line.

Negative cell control: NIH/3T3 (PMID: 9405066; PMID:15143067).

Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red). The nuclear counter stain is DAPI (blue). The negative control is the secondary antibody only.
Flow Cytometry - Anti-PC1/3 antibody [EPR21908] (ab220363)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cell line labeling PC1/3 with ab220363 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-PC1/3 antibody [EPR21908] (ab220363)

PC1/3 was immunoprecipitated from 0.35 mg Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate with ab220363 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220363 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate 10 µg (Input).
Lane 2: ab220363 IP in Beta-TC-6 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220363 in Beta-TC-6 whole cell lysate (-).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.

The PC1/3 protein undergoes multiple intracellular cleavage steps to its 66 kDa mature form (PMID: 26778167).
Anti-PC1/3 antibody [EPR21908] (ab220363)

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