Anti-PC1/3 antibody (ab3532)
Key features and details
- Rabbit polyclonal to PC1/3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-PC1/3 antibody
See all PC1/3 primary antibodies -
Description
Rabbit polyclonal to PC1/3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse PC1/3 aa 100-200.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PC1/3 antibody (ab3532)Western blot detection of PC1/3 from mouse pituitary extract using ab3532.
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Western blot - Anti-PC1/3 antibody (ab3532)Anti-PC1/3 antibody (ab3532) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 87 + 68 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
PC1/3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of PC1/3 due to the presence of a 27 amino acid signal peptide and an 83 amino acid propeptide.
