Anti-PC1/3 antibody (ab191452)
Key features and details
- Rabbit polyclonal to PC1/3
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PC1/3 antibody
See all PC1/3 primary antibodies -
Description
Rabbit polyclonal to PC1/3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human PC1/3 aa 700 to the C-terminus (C terminal).
Database link: P29120 -
Positive control
- Rat Liver Lysate, Rat Thymus Lysate, A549 Cell Lysate, HELA Cell Lysate, COLO320 Cell Lysate & PANC Cell Lysate, Rat Intestine Tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of PC1/3 using ab191452 in human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab191452 overnight at 4°C. DyLight®488 Conjugated Avidin was used and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat Intestine tissue labeling PC1/3 with ab191452 at 1 µg/ml.
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All lanes : Anti-PC1/3 antibody (ab191452) at 0.5 µg/ml
Lane 1 : Rat Liver Tissue Lysate
Lane 2 : Rat Thymus Tissue Lysate
Lane 3 : A549 cell lysate
Lane 4 : HELA cell lysate
Lane 5 : COLO320 cell lysate
Lane 6 : PANC cell lysate
Predicted band size: 84 kDa