Anti-CRAT antibody (ab153750)
Key features and details
- Rabbit polyclonal to CRAT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CRAT antibody
See all CRAT primary antibodies -
Description
Rabbit polyclonal to CRAT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to a region within amino acids 388-609 of Human CRAT.
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Positive control
- 293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CRAT antibody (ab153750)Anti-CRAT antibody (ab153750) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 71 kDa
7.5% SDS PAGE -
Western blot - Anti-CRAT antibody (ab153750) This image is courtesy of an anonymous AbreviewAll lanes : Anti-CRAT antibody (ab153750) at 1/1000 dilution
Lane 1 : Wildtype C57BL/6J quadriceps muscle whole cell lysate
Lane 2 : CrAT Knockout C57BL/6J quadriceps muscle whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alexa Fluor® 680-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa (possible non-specific binding), 37 kDa (possible non-specific binding)
Exposure time: 1 minuteThe CrAT knockout was achieved in C57BL6/J transgenic animals expressing a tamoxifen-inducible Cre whose CrAT alleles were homozygously floxed. This model was previously validated through genome sequencing, mRNA expression, and protein expression
• Western Membrane: Nitrocellulose 0.45µm
• Lysis buffer: 20mM Tris-HCl, 4% SDS, 10mM NaF, 1mM EDTA, 20% glycerol
