Anti-PKM antibody (ab137791)
Key features and details
- Rabbit polyclonal to PKM
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PKM antibody
See all PKM primary antibodies -
Description
Rabbit polyclonal to PKM -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human PKM aa 243-531.
Database link: P14618 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PKM antibody (ab137791)All lanes : Anti-PKM antibody (ab137791) at 1/5000 dilution
Lane 1 : Non-transfected (-) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T whole cell extracts transfected (+) with PKM shRNA
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 58 kDa7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (ab137791)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue staining PKM protein at cytosol with ab137791 at 1/500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunoprecipitation - Anti-PKM antibody (ab137791)Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labelling Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytoplasm (green) with ab137791 at 1/500 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/500 dilution. Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-PKM antibody (ab137791)Immunoprecipitation of PKM2 protein from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate using 5 μg of ab137791.
IP Sample:
1- HEK-293T cells
2- Control IgG
3- ab137791
Western blot analysis was performed using ab137791, followed by anti-Rabbit IgG antibody. -
Western blot - Anti-PKM antibody (ab137791)All lanes : Anti-PKM antibody (ab137791) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 58 kDa7.5% SDS-PAGE
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Western blot - Anti-PKM antibody (ab137791)Mouse brain tissue lysate at 50 µg
Predicted band size: 58 kDa7.5 % SDS-PAGE
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Western blot - Anti-PKM antibody (ab137791)Anti-PKM antibody (ab137791) at 1/2000 dilution + Rat tissue lysate at 50 µg
Predicted band size: 58 kDa7.5 % SDS-PAGE
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Western blot - Anti-PKM antibody (ab137791)All lanes : Anti-PKM antibody (ab137791) at 1/5000 dilution
Lane 1 : Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected (+) HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 58 kDa7.5% SDS-PAGE
