Anti-Artemin antibody [EPR9763] - BSA and Azide free (ab250054)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9763] to Artemin - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Artemin antibody [EPR9763] - BSA and Azide free
See all Artemin primary antibodies -
Description
Rabbit monoclonal [EPR9763] to Artemin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: T-47D, NIH/3T3 and PC-12 whole cell lysate. Mouse and rat brain lysate; PC-12 whole cell lysate. IP: PC-12 whole cell lysate
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General notes
Ab250054 is the carrier-free version of ab178434. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250054 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9763 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab178434, the same antibody clone in a different buffer formulation.
Purified ab178434 at 1/30 dilution (2µg) immunoprecipitating Artemin in PC-12 whole cell lysate.
Lane 1 (input): PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate 10µg
Lane 2 (+): ab178434 + PC-12 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178434 in PC-12 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa -
All lanes : Anti-Artemin antibody [EPR9763] (ab178434) at 1/1000 dilution
Lane 1 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : Rat brain lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDaThis data was developed using ab178434, the same antibody clone in a different buffer formulation.
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