Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13574(B)-30] to SMYD1
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-SMYD1 antibody [EPR13574(B)-30]
See all SMYD1 primary antibodies
Description

Rabbit monoclonal [EPR13574(B)-30] to SMYD1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SMYD1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8NB12
Positive control
- Human skeletal muscle and fetal heart lysates; Human heart tissue and skeletal muscle tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13574(B)-30
Isotype

IgG
Research areas

Western blot - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

All lanes : Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372) at 1/1000 dilution

Lane 1 : Human fetal heart lysates
Lane 2 : Human skeletal muscle lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SMYD1 with ab181372 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SMYD1 with ab181372 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal heart lysate or (Lane 2) 1XPBS (negative control) immunoprecipitated using ab181372 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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