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Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13574(B)-30] to SMYD1
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SMYD1 antibody [EPR13574(B)-30]
    See all SMYD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13574(B)-30] to SMYD1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SMYD1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8NB12

  • Positive control

    • Human skeletal muscle and fetal heart lysates; Human heart tissue and skeletal muscle tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13574(B)-30

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)
    Western blot - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)
    All lanes : Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372) at 1/1000 dilution

    Lane 1 : Human fetal heart lysates
    Lane 2 : Human skeletal muscle lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SMYD1 with ab181372 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SMYD1 with ab181372 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)
    Immunoprecipitation - Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

    Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal heart lysate or (Lane 2) 1XPBS (negative control) immunoprecipitated using ab181372 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)
    Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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