Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-MYO1C antibody [EPR14771] (ab194828)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14771] to MYO1C
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-MYO1C antibody [EPR14771]
See all MYO1C primary antibodies
Description

Rabbit monoclonal [EPR14771] to MYO1C
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- A431, HT1080, 293T cell lysates; mouse heart and rat heart lysates; Human tonsil tissue; A431 cells.
General notes
This product was previously labelled as Myosin 1C

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14771
Isotype

IgG
Research areas

Western blot - Anti-MYO1C antibody [EPR14771] (ab194828)

All lanes : Anti-MYO1C antibody [EPR14771] (ab194828) at 1/20000 dilution

Lane 1 : A431 cell lysate
Lane 2 : HT1080 cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 122 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1C antibody [EPR14771] (ab194828)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYO1C with ab194828 at 1/200 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab194828. Cytoplasm staining on Human tonsil was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MYO1C antibody [EPR14771] (ab194828)

Immunofluorescent analysis of A431 cells labeling MYO1C with ab194828 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm and nuclear on A431 cell line was observed.
Western blot - Anti-MYO1C antibody [EPR14771] (ab194828)

All lanes : Anti-MYO1C antibody [EPR14771] (ab194828) at 1/2000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 122 kDa
Flow Cytometry - Anti-MYO1C antibody [EPR14771] (ab194828)

Flow cytometry analysis of HeLa cells labelling MYO1C (red) with purified ab194828 at dilution of 1/80. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Anti-MYO1C antibody [EPR14771] (ab194828)

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