Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYO1C antibody [EPR14771] (ab194828)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14771] to MYO1C
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MYO1C antibody [EPR14771]
    See all MYO1C primary antibodies

  • Description

    Rabbit monoclonal [EPR14771] to MYO1C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431, HT1080, 293T cell lysates; mouse heart and rat heart lysates; Human tonsil tissue; A431 cells.

  • General notes

     

     

     This product was previously labelled as Myosin 1C

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14771

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MYO1C antibody [EPR14771] (ab194828)
    Western blot - Anti-MYO1C antibody [EPR14771] (ab194828)
    All lanes : Anti-MYO1C antibody [EPR14771] (ab194828) at 1/20000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HT1080 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 122 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1C antibody [EPR14771] (ab194828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1C antibody [EPR14771] (ab194828)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYO1C with ab194828 at 1/200 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194828. Cytoplasm staining on Human tonsil was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MYO1C antibody [EPR14771] (ab194828)
    Immunocytochemistry/ Immunofluorescence - Anti-MYO1C antibody [EPR14771] (ab194828)

    Immunofluorescent analysis of A431 cells labeling MYO1C with ab194828 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm and nuclear on A431 cell line was observed.

  • Western blot - Anti-MYO1C antibody [EPR14771] (ab194828)
    Western blot - Anti-MYO1C antibody [EPR14771] (ab194828)
    All lanes : Anti-MYO1C antibody [EPR14771] (ab194828) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 122 kDa

  • Flow Cytometry - Anti-MYO1C antibody [EPR14771] (ab194828)
    Flow Cytometry - Anti-MYO1C antibody [EPR14771] (ab194828)

    Flow cytometry analysis of HeLa cells labelling MYO1C (red) with purified ab194828 at dilution of 1/80. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Anti-MYO1C antibody [EPR14771] (ab194828)
    Anti-MYO1C antibody [EPR14771] (ab194828)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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