Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17322-9] to MYOM1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free
    See all MYOM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17322-9] to MYOM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251439 is the carrier-free version of ab205618.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution + Human fetal heart lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 188 kDa
    Observed band size: 188 kDa


    Exposure time: 2 minutes


    This data was developed using ab205618, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    All lanes : Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 188 kDa
    Observed band size: 188 kDa


    Exposure time: 5 seconds


    This data was developed using ab205618, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    All lanes : Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution

    Lane 1 : Rat heart tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

    Predicted band size: 188 kDa
    Observed band size: 188 kDa


    Exposure time: 5 seconds


    This data was developed using ab205618, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    This data was developed using ab205618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human cardiac muscle tissue is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    This data was developed using ab205618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on human tonsil tissue is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    This data was developed using ab205618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    This data was developed using ab205618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
    Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)

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