Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (mutated D614G) (His tag) (ab281479)
Key features and details
- Expression system: CHO cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Size Exclusion Chromatography
-
Product name
Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (mutated D614G) (His tag)
See all SARS-CoV-2 spike glycoprotein proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Endotoxin level
Expression system
CHO cellsProtein length
Protein fragmentAnimal free
YesNature
Recombinant-
Species
Human coronavirus -
Sequence
N-terminus VNLT -
Predicted molecular weight
400 kDa including tags -
Modifications
mutated D614G -
Tags
His tag C-Terminus -
Additional sequence information
C-terminal transmembrane region is replaced with a trimerization domain and His-tag. Accession: MN908947. Strain: SARS-CoV-2 Betacoronavirus, Wuhan-Hu-1.
Specifications
Our Abpromise guarantee covers the use of ab281479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Size Exclusion Chromatography
-
Form
Liquid -
Additional notes
Contains two stabilizing proline mutations and scrambled S1/S2 furin cleavage site (Wrapp et al., 2020).
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 99% PBS
Images
-
SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (His tag) (ab281479)
SDS-PAGE of ab281479
-
Size Exclusion Chromatography - Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (His tag) (ab281479)Verification of purity and size of ab281479 under nondenaturing conditions. Size-exclusion chromatography (SEC) plot with peak at 4.0 minutes, corresponding to a size of ~400kD.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab281479 has not yet been referenced specifically in any publications.
Preparation and Storage
Images
-
SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (His tag) (ab281479)
SDS-PAGE of ab281479
-
Size Exclusion Chromatography - Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (His tag) (ab281479)
Verification of purity and size of ab281479 under nondenaturing conditions. Size-exclusion chromatography (SEC) plot with peak at 4.0 minutes, corresponding to a size of ~400kD.