Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)
Key features and details
- Rabbit polyclonal to SARS-CoV-2 spike glycoprotein - Coronavirus
- Suitable for: IHC-P, ELISA, ICC, WB
- Isotype: IgG
Overview
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Product name
Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus
See all SARS-CoV-2 spike glycoprotein primary antibodies -
Description
Rabbit polyclonal to SARS-CoV-2 spike glycoprotein - Coronavirus -
Host species
Rabbit -
Specificity
The immunogen for ab272504 is within the last 50 aa of the spike protein - a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The Extracellular domain (ECD) is from aa 1 to 1208 (full length 1273aa). Therefore, this antibody detects the transmembrane and cytoplasm domains at the C terminus, but does not detect the ECD (which is the region expressed in many commercially available spike proteins). ab272504 can be used for the detection of full length spike protein and spike protein in COVID-19 patient samples. It will detect 4 ng of free peptide at 1 μg/mL.
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Tested applications
Suitable for: IHC-P, ELISA, ICC, WBmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
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Positive control
- WB: HEK-293 cells overexpressing SARS-CoV-2 spike glycoprotein, cell lysate. ICC: HEK-293 cells overexpressing SARS-CoV-2 spike glycoprotein. IHC-P: COVID-19 patient lung tissue.
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General notes
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ab272504, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung but not in non-COVID-19 patient lung.
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Immunocytochemistry - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 spike transfected cells labelling SARS-CoV-2 (Covid-19) Spike with ab272504 at 1 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)All lanes : Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) at 1 µg/ml
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : HEK-293 overexpressing SARS-CoV-2 spike glycoprotein, cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incubation for 1 hour at room temperature.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ab272504, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages of COVID-19 patient lung.
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ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)A direct ELISA was performed using antigen or control peptide as coating antigen and ab272504 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 0.5 ng/ml to 1000 ng/ml.