Recombinant Human coronavirus SARS Nucleocapsid Protein (His tag) (ab279288)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: Sandwich ELISA, SDS-PAGE
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Product name
Recombinant Human coronavirus SARS Nucleocapsid Protein (His tag)
See all SARS Nucleocapsid Protein proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Sequence
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTA SWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGK MKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRN PANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPG SSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKS AAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH WPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQV ILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADL DDFSKQLQQSMSSADSTQARNPANNAAIVLQLPQGTTLPKGFYAEGSRGG SQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQ LESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGP EQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGT WLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADET QALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA -
Predicted molecular weight
47 kDa including tags -
Actual molecular weight
47 kDa including tags -
Amino acids
1 to 419 -
Tags
His tag C-Terminus -
Additional sequence information
Sequence Strain: Wuhan-Hu-1
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Preparation and Storage
- N
- NC
- Nucleocapsid protein
Images
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SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab279288)
SDS-PAGE analysis of ab279288 (2.5 μg).
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Sandwich ELISA - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab279288)sELISA analysis of threee different batches of ab279288.
Capture antibody is a Mouse Anti-SARS Coronavirus Nucleaoprotein antibody, detection antibody is a Mouse Anti-SARS Coronavirus Nucleaoprotein antibody-HRP conjugate.
Plates were coated overnight with capture antibody (5 μg/ml) in DPB, 100 µl/well at 23°. Blocked with 1% BSA in DPBS (300 µl/well for 1.5 hours. Washed X3 with PBS + 0.1% Tween 20. Diluted antigen (ab279288) added in DPBS/1% BSA/0.1% Tween 20 at 100 µl/well and incubated for 3.5 hours at 23°C. Washed X3 , as above. Detection antibody (1 μg/ml) added in DPBS/1% BSA/0.1% Tween 20 at 100 μl/well and incubated with shaking for 2 hours at 23°C. Detection with TMB (100 μl/well). Incubated for 10 minutes. Read absorbance at 450/620nm.
