Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
Key features and details
- Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KDM1/LSD1 antibody - Nuclear Marker
See all KDM1/LSD1 primary antibodies -
Description
Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human KDM1/LSD1 (N terminal). The exact sequence is proprietary. ab37165 was raised against a peptide corresponding to 17 amino acids near the N terminus of human LSD1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)All lanes : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 0.2 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 5 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 10 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 11 : SK-N-SH (human neuroblastoma cell line) cell lysate
Lane 12 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 13 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 14 : YB2/0 (rat spleen lymphoblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 93 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Diluting buffer and concentration: 1 hour at room temperature in 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)IHC image of KDM1/LSD1 staining in human prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37165, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)All lanes : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 0.2 µg/ml
Lane 1 : Mouse Testis tissue lysate
Lane 2 : Mouse Spleen tissue lysate
Lane 3 : Mouse Kidney tissue lysate
Lane 4 : Mouse Colon tissue lysate
Lane 5 : Mouse Stomach tissue lysate
Lane 6 : Mouse Brain tissue lysate
Lane 7 : Mouse Skin tissue lysate
Lane 8 : Mouse Skeletal Muscle tissue lysate
Lane 9 : Mouse Bladder tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 93 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Diluting buffer and concentration: 1 hour at room temperature in 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human breast carcinoma tissue labeling KDM1/LSD1 with ab37165 at 2μg/ml.
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)Lane 1 : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 1 µg/ml
Lane 2 : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 2 µg/ml
All lanes : Mouse P815 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 93 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
