Antibodies, Proteins, Kits and Reagents for Life Science

Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

Key features and details

Mouse monoclonal [1B2E5 + 1B2F2] to KDM1/LSD1
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human
Isotype: IgG1

Product name

Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2]
See all KDM1/LSD1 primary antibodies
Description

Mouse monoclonal [1B2E5 + 1B2F2] to KDM1/LSD1
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human KDM1/ LSD1.
Positive control
- HeLa and Jurkat cell lysate for Western blot. Human kidnay and lung carcinoma tissue for Immunohistochemistry.
General notes

Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

1B2E5 + 1B2F2
Isotype

IgG1
Research areas

Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: KDM1/LSD1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab31954 observed at 110 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab31954 was shown to specifically recognize KDM1/LSD1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when KDM1/LSD1 knockout samples were used. Wild-type and KDM1/LSD1 knockout samples were subjected to SDS-PAGE. ab31954 and ab181602 (loading control to GAPDH) were diluted 1/250 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.
Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

All lanes : Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

Lane 1 : Molecular weight marker
Lane 2 : LSD1 recombinant protein (NB: truncated form)
Lane 3 : HeLa cell lysate

Predicted band size: 93 kDa

Primary antibody dilution range 1:500-1:2000.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.

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