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Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

Key features and details

  • Mouse monoclonal [1B2E5 + 1B2F2] to KDM1/LSD1
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2]
    See all KDM1/LSD1 primary antibodies

  • Description

    Mouse monoclonal [1B2E5 + 1B2F2] to KDM1/LSD1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human KDM1/ LSD1.

  • Positive control

    • HeLa and Jurkat cell lysate for Western blot. Human kidnay and lung carcinoma tissue for Immunohistochemistry.

  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    1B2E5 + 1B2F2

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
    Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: KDM1/LSD1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab31954 observed at 110 kDa. Red - loading control, ab181602, observed at 37 kDa.


    ab31954 was shown to specifically recognize KDM1/LSD1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when KDM1/LSD1 knockout samples were used. Wild-type and KDM1/LSD1 knockout samples were subjected to SDS-PAGE. ab31954 and ab181602 (loading control to GAPDH) were diluted 1/250 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.
  • Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
    Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
    All lanes : Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

    Lane 1 : Molecular weight marker
    Lane 2 : LSD1 recombinant protein (NB: truncated form)
    Lane 3 : HeLa cell lysate

    Predicted band size: 93 kDa



    Primary antibody dilution range 1:500-1:2000.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
    Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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