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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation Lysine demethylation

Anti-KDM1/LSD1 antibody (ab62582)

Anti-KDM1/LSD1 antibody (ab62582)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to KDM1/LSD1
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KDM1/LSD1 antibody
    See all KDM1/LSD1 primary antibodies
  • Description

    Rabbit polyclonal to KDM1/LSD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic 16 amino acid peptide from near the centre of human KDM1/ LSD1.

  • Positive control

    • WB: MCF-7, Jurkat, and P815 cell lysates. IHC-P: Human small intestine tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation

Images

  • Western blot - Anti-KDM1/LSD1 antibody (ab62582)
    Western blot - Anti-KDM1/LSD1 antibody (ab62582)
    All lanes : Anti-KDM1/LSD1 antibody (ab62582) at 1 µg/ml

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution

    Predicted band size: 93 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody (ab62582)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody (ab62582)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using ab62582 at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody (ab62582)
    Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody (ab62582)
    Immunofluorescence of LSD1 in Human Small Intestine cells using ab62582 at 20 ug/ml.

  • Western blot - Anti-KDM1 / LSD1 antibody (ab62582)
    Western blot - Anti-KDM1 / LSD1 antibody (ab62582)
    Lane 1 : Anti-KDM1/LSD1 antibody (ab62582) at 1 µg/ml
    Lane 2 : Anti-KDM1/LSD1 antibody (ab62582) at 2 µg/ml

    All lanes : P815 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 93 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?
    Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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