Anti-KDM1/LSD1 antibody (ab62582)
Key features and details
- Rabbit polyclonal to KDM1/LSD1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KDM1/LSD1 antibody
See all KDM1/LSD1 primary antibodies -
Description
Rabbit polyclonal to KDM1/LSD1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic 16 amino acid peptide from near the centre of human KDM1/ LSD1.
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Positive control
- WB: MCF-7, Jurkat, and P815 cell lysates. IHC-P: Human small intestine tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-KDM1/LSD1 antibody (ab62582) at 1 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 93 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human small intestine tissue using ab62582 at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence of LSD1 in Human Small Intestine cells using ab62582 at 20 ug/ml.
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Lane 1 : Anti-KDM1/LSD1 antibody (ab62582) at 1 µg/ml
Lane 2 : Anti-KDM1/LSD1 antibody (ab62582) at 2 µg/ml
All lanes : P815 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 93 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.