Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DOCK8 antibody [EPR12511] (ab175208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12511] to DOCK8
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-DOCK8 antibody [EPR12511]
    See all DOCK8 primary antibodies

  • Description

    Rabbit monoclonal [EPR12511] to DOCK8

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human DOCK8. The exact sequence is proprietary.
    Database link: Q8NF50

  • Positive control

    • Raji and THP1 cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12511

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)
    Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)

    Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling DOCK8 with purified ab175208 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-DOCK8 antibody [EPR12511] (ab175208)
    Western blot - Anti-DOCK8 antibody [EPR12511] (ab175208)
    All lanes : Anti-DOCK8 antibody [EPR12511] (ab175208) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 239 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)
    Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)

    Immunofluorescent staining of Raji cells labeling DOCK8 with ab175208 at 1/100 dilution (green). DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-DOCK8 antibody [EPR12511] (ab175208)
    Flow Cytometry - Anti-DOCK8 antibody [EPR12511] (ab175208)

    Flow cytometric analysis of permeabilized THP1 cells labeling DOCK8 with ab175208 at 1/10 dilution (red) compared to a Rabbit IgG (green).

  • Anti-DOCK8 antibody [EPR12511] (ab175208)
    Anti-DOCK8 antibody [EPR12511] (ab175208)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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