Anti-DOCK8 antibody [EPR12511] (ab175208)
![Anti-DOCK8 antibody [EPR12511] (ab175208)](https://www.abcam.com/ps/products/175/ab175208/Images/ab175208-261225-anti-dock8-antibody-epr12511-immunofluorescence.jpg "Anti-DOCK8 antibody [EPR12511] (ab175208)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12511] to DOCK8
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-DOCK8 antibody [EPR12511]
See all DOCK8 primary antibodies -
Description
Rabbit monoclonal [EPR12511] to DOCK8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human DOCK8. The exact sequence is proprietary.
Database link: Q8NF50 -
Positive control
- Raji and THP1 cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12511 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)
Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling DOCK8 with purified ab175208 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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![Western blot - Anti-DOCK8 antibody [EPR12511] (ab175208)](https://www.abcam.com/ps/products/175/ab175208/Images/ab175208-201709-anti-dock8-antibody-epr12511-western-blot.jpg)
Western blot - Anti-DOCK8 antibody [EPR12511] (ab175208)All lanes : Anti-DOCK8 antibody [EPR12511] (ab175208) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 239 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)](https://www.abcam.com/ps/products/175/ab175208/Images/ab175208-201710-anti-dock8-antibody-epr12511-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (ab175208)Immunofluorescent staining of Raji cells labeling DOCK8 with ab175208 at 1/100 dilution (green). DAPI nuclear staining (blue).
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![Flow Cytometry - Anti-DOCK8 antibody [EPR12511] (ab175208)](https://www.abcam.com/ps/products/175/ab175208/Images/ab175208-201711-anti-dock8-antibody-epr12511-flow-cytometry.jpg)
Flow Cytometry - Anti-DOCK8 antibody [EPR12511] (ab175208)Flow cytometric analysis of permeabilized THP1 cells labeling DOCK8 with ab175208 at 1/10 dilution (red) compared to a Rabbit IgG (green).
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![Anti-DOCK8 antibody [EPR12511] (ab175208)](https://www.abcam.com/ps/products/175/ab175208/Images/ab175208-5-benefits-of-recombinant-antibodies.png)
Anti-DOCK8 antibody [EPR12511] (ab175208)
