Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12511] to DOCK8 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-DOCK8 antibody [EPR12511] - BSA and Azide free
    See all DOCK8 primary antibodies

  • Description

    Rabbit monoclonal [EPR12511] to DOCK8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human DOCK8. The exact sequence is proprietary.
    Database link: Q8NF50

  • General notes

    Ab240182 is the carrier-free version of ab175208. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240182 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)
    Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)

    Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling DOCK8 with purified ab175208 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175208).

  • Flow Cytometry - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)
    Flow Cytometry - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)

    Flow cytometric analysis of permeabilized THP1 cells labeling DOCK8 with ab175208 at 1/10 dilution (red) compared to a Rabbit IgG (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175208).

  • Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)
    Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)

    Immunofluorescent staining of Raji cells labeling DOCK8 with ab175208 at 1/100 dilution (green). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175208).

  • Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)
    Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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