Anti-17-beta-Estradiol antibody [EPPTX-R2] (ab215528)
![Anti-17-beta-Estradiol antibody [EPPTX-R2] (ab215528)](https://www.abcam.com/ps/products/215/ab215528/Images/ab215528-270574-anti-17-beta-estradiol-antibody-epptx-r2-competitive-elisa.jpg "Anti-17-beta-Estradiol antibody [EPPTX-R2] (ab215528)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R2] to 17-beta-Estradiol
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Overview
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Product name
Anti-17-beta-Estradiol antibody [EPPTX-R2]
See all 17-beta-Estradiol primary antibodies -
Description
Rabbit monoclonal [EPPTX-R2] to 17-beta-Estradiol -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Human 17-beta-Estradiol. Immunogen is Estradiol-6-CMO-BSA.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R2 -
Isotype
IgG
Images
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Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] (ab215528)
Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of 17β-Estradiol (0, 1, 2, 4, 8, 16, 32 pg/ml) was added (75 μl). Then 3-5 ng/ml of ab215528 (25 μl) was added followed by incubation by shaking for 60 minutes at room temperature. Then 25 μl of HRP-conjugated 17β-Estradiol was added and incubated for 60 minutes at room temperature. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 30 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] (ab215528)](https://www.abcam.com/ps/products/215/ab215528/Images/ab215528-270573-anti-17-beta-estradiol-antibody-epptx-r2-competitive-elisa.jpg)
Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] (ab215528)ab215528 is specific to 17β-Estradiol and the percent cross reactivity with other compounds is demonstrated in the table.
The data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Anti-17-beta-Estradiol antibody [EPPTX-R2] (ab215528)](https://www.abcam.com/ps/products/215/ab215528/Images/ab215528-3-benefits-of-recombinant-antibodies.png)
Anti-17-beta-Estradiol antibody [EPPTX-R2] (ab215528)
