Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)
![Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)](https://www.abcam.com/ps/products/251/ab251114/Images/ab251114-415174-anti-smug1-antibody-epr15624-bsa-and-azide-free-western-blot-hap1-wt-hap1-smug1-knockout-molt4-hek293.jpg "Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15624] to SMUG1 - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SMUG1 antibody [EPR15624] - BSA and Azide free
See all SMUG1 primary antibodies -
Description
Rabbit monoclonal [EPR15624] to SMUG1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, 293 and MOLT4 cell lysates.
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General notes
Ab251114 is the carrier-free version of ab192240. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251114 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15624 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SMUG1 knockout HAP1 cell lysate
Lane 3 : MOLT-4 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab192240).
Lanes 1 - 4: Merged signal (red and green). Green - ab192240 observed at 30 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab192240 was shown to react with SMUG1 in wild-type HAP1 cells in western blot with loss of signal observed in SMUG1 knockout sample. ild-type and SMUG1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab192240 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)](https://www.abcam.com/ps/products/251/ab251114/Images/ab251114-2-anti-smug1-antibody-epr15624-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)This data was developed using ab192240, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab192240 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Western blot - Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)](https://www.abcam.com/ps/products/251/ab251114/Images/ab251114-399384-anti-smug1-antibody-epr15624-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/5000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDaThis data was developed using ab192240, the same antibody clone in a different buffer formulation.
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![Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)](https://www.abcam.com/ps/products/251/ab251114/Images/ab251114-4-benefits-of-recombinant-antibodies.png)
Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (ab251114)
