Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRK3 antibody [EPR2041(2)] - BSA and Azide free (ab247828)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2041(2)] to GRK3 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GRK3 antibody [EPR2041(2)] - BSA and Azide free
    See all GRK3 primary antibodies

  • Description

    Rabbit monoclonal [EPR2041(2)] to GRK3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human hepatocellular carcinoma and brain tissue. ICC/IF: Jurkat cells.

  • General notes

    ab247828 is the carrier-free version of ab109303 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247828 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK3 antibody [EPR2041(2)] - BSA and Azide free (ab247828)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK3 antibody [EPR2041(2)] - BSA and Azide free (ab247828)
    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling GRK3 with Purified ab109303 at 1:50 dilution (4.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109303)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] - BSA and Azide free (ab247828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] - BSA and Azide free (ab247828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling GRK3 with Purified ab109303 at 1:500 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109303)
  • Anti-GRK3 antibody [EPR2041(2)] - BSA and Azide free (ab247828)
    Anti-GRK3 antibody [EPR2041(2)] - BSA and Azide free (ab247828)

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