Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)
![Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)](https://www.abcam.com/ps/products/157/ab157186/Images/ab157186-164333-anti-dcdc2-antibody-epr11126b-western-blot.jpg "Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11126(B)] to DCDC2/RU2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-DCDC2/RU2 antibody [EPR11126(B)]
See all DCDC2/RU2 primary antibodies -
Description
Rabbit monoclonal [EPR11126(B)] to DCDC2/RU2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human DCDC2/RU2.
Database link: Q9UHG0 -
Positive control
- Fetal brain, HeLa and 293T lysates; Human kidney and pancreas tissues; HeLa cells; 293T cells; Immunoprecipitation pellet from fetal brain lysate.
-
General notes
This product was previously labelled as DCDC2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11126(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)All lanes : Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186) at 1/1000 dilution
Lanes 1 & 4 : Fetal brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)](https://www.abcam.com/ps/products/157/ab157186/Images/ab157186-164332-anti-dcdc2-antibody-epr11126b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCDC2/RU2 with ab157186 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)](https://www.abcam.com/ps/products/157/ab157186/Images/ab157186-164331-anti-dcdc2-antibody-epr11126b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DCDC2/RU2 with ab157186 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)](https://www.abcam.com/ps/products/157/ab157186/Images/ab157186-164330-anti-dcdc2-antibody-epr11126b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)Immunofluorescent analysis of HeLa cells labeling DCDC2/RU2 with ab157186 at 1/250 dilution.
-
![Flow Cytometry - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)](https://www.abcam.com/ps/products/157/ab157186/Images/ab157186-164329-anti-dcdc2-antibody-epr11126b-flow-cytometry.jpg)
Flow Cytometry - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)Flow cytometric analysis of permeabilized 293T cells labeling DCDC2/RU2 with ab157186 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
-
![Western blot - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)](https://www.abcam.com/ps/products/157/ab157186/Images/ab157186-164328-anti-dcdc2-antibody-epr11126b-western-blot.jpg)
Western blot - Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186) at 1/1000 dilution + immunoprecipitation pellet from fetal brain lysate
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
-
![Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)](https://www.abcam.com/ps/products/157/ab157186/Images/ab157186-7-benefits-of-recombinant-antibodies.png)
Anti-DCDC2/RU2 antibody [EPR11126(B)] (ab157186)
