Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (ab224259)
![Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (ab224259)](https://www.abcam.com/ps/products/224/ab224259/Images/ab224259-284387-anti-17-beta-estradiol-antibody-epptx-r2-competitive-elisa.jpg "Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (ab224259)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R2] to 17-beta-Estradiol - BSA and Azide free
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Overview
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Product name
Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free
See all 17-beta-Estradiol primary antibodies -
Description
Rabbit monoclonal [EPPTX-R2] to 17-beta-Estradiol - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule. Immunogen is Estradiol-6-CMO-BSA.
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General notes
Ab224259 is the carrier-free version of ab215528. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab224259 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R2 -
Isotype
IgG
Images
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Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (ab224259)
ab224259 is specific to 17β-Estradiol and the percent cross reactivity with other compounds is demonstrated in the table.
The data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (ab224259)](https://www.abcam.com/ps/products/224/ab224259/Images/ab224259-284386-anti-17-beta-estradiol-antibody-epptx-r2-competitive-elisa.jpg)
Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (ab224259)This ELISA data used the same anti-17-beta-Estradiol antibody clone [EPPTX-R2] in a different buffer formulation (cat# ab215528).
Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of 17β-Estradiol (0, 1, 2, 4, 8, 16, 32 pg/ml) was added (75 μl). Then 3-5 ng/ml of ab215528 (25 μl) was added followed by incubation by shaking for 60 minutes at room temperature. Then 25 μl of HRP-conjugated 17β-Estradiol was added and incubated for 60 minutes at room temperature. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 30 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (ab224259)](https://www.abcam.com/ps/products/224/ab224259/Images/ab224259-3-benefits-of-recombinant-antibodies.png)
Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (ab224259)
