Senescence Assay Kit (Beta Galactosidase, Fluorescence) (ab228562)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Senescence Assay Kit (Beta Galactosidase, Fluorescence)
See all Senescence-associated beta Galactosidase kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Product overview
Senescence Assay Kit (Beta Galactosidase, Fluorescence) (ab228562) is designed to fluorescently detect senescence-associated Beta Galactosidase activity in cultured cells by flow cytometry. The senescence-associated Beta Galactosidase is present only in senescent cells and is not found in pre-senescent, quiescent or immortal cells.
See Senescence Assay Kit ab65351 to stain for beta galactosidase activity in tissues and cell cultures with detection by microscopy.
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Notes
Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of Beta Galactosidase activity, and altered patterns of gene expression.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Senescence Dye 1 x 150µl Wash Buffer 2 x 100ml -
Research areas
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Relevance
Cellular senescence is a growth-arrest program by which normal diploid cells lose the ability to divide, and it plays a critical role in regulating lifespan both in vivo and in vitro. Cellular senescence occurs as reflection of organism aging and in response to internal and external stress signals.
Images
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Typical Data
NIH/3T3 (mouse embryo fibroblast cell line) cells were plated at 5 x 105 cells per well in a 24 well, treated for 4 hours (with and without 200 nM of Daunorubicin HCl; test and control response respectively) in complete cell culture media for 48 hours at 37°C/5% CO2. Media was removed and replaced with media containing Senescence Dye and incubated for 2 hours at 37°C/5% CO2. After incubation time, cells were washed 2x with Wash Buffer, the cells were trypsinized, washed once with Wash Buffer and analyzed by FACS.
