COVID-19 Spike Protein ELISA Kit (ab274342)
Key features and details
- Sensitivity: 2.7 ng/ml
- Range: 2.744 ng/ml - 2000 ng/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
Overview
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Product name
COVID-19 Spike Protein ELISA Kit
See all COVID-19 Spike Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum -
Assay type
Quantitative -
Sensitivity
2.7 ng/ml -
Range
2.744 ng/ml - 2000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture media 110 105% - 117% -
Assay duration
Multiple steps standard assay -
Product overview
COVID-19 Spike Protein ELISA Kit (ab274342) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of the SARS-CoV-2 Spike Protein, subunit S2 in serum (plasma is not recommended in this assay) and cell culture supernatants.
This assay employs an antibody specific for COVID-19 Spike Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and COVID-19 Spike Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-COVID-19 Spike Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID-19 Spike Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Antibody 2 vials 100X HRP-Streptavidin Concentrate 1 x 200µl Lyophilized Protein Standard 2 vials Microplate 1 unit Stop Solution 1 x 8ml TMD Substrate Solution 1 x 12ml -
Research areas
Images
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COVID -19 Spike Protein Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.