Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)
![Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.com/ps/products/281/ab281301/Images/ab281301-456639-SARSCoV2-nucleocapsid-protein-antibody-HL5410-wb.JPG "Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL5410] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
- Suitable for: WB, ICC, Sandwich ELISA, Indirect ELISA, IHC-P
Overview
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Product name
Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free
See all SARS-CoV-2 nucleocapsid protein primary antibodies -
Description
Rabbit monoclonal [HL5410] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, Sandwich ELISA, Indirect ELISA, IHC-Pmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1
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Positive control
- WB: SARS-CoV Nucleocapsid transfected 293T whole cell extracts, SARS-CoV Nucleocapsid transfected Vero E6 cells. ICC: SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cells. IHC-P: SARS-CoV Nucleocapsid transfected 293T cells. ELISA: Recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL5410 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) extracts
Lane 2 : SARS-CoV NP transfected 293T whole cell extracts
Lane 3 : SARS-CoV-2 (COVID-19) transfected 293T whole cell extracts
Lane 4 : MERS-CoV NP transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281301)Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281301 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.
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![Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.com/ps/products/281/ab281301/Images/ab281301-5-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-immunocytochemistry-293tcells.jpg)
Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281301 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).
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![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.com/ps/products/281/ab281301/Images/ab281301-457465-SARSCoV2-nucleocapsid-protein-antibody-HL5410-elisa-3.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Sandwich ELISA detection of non-transfected and SARS-CoV-2, MERS-CoV and SARS-CoV Nucleocapsid transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using ab281301 as capture antibody at concentration of 5 μg/mL and a SARS-CoV-2 (COVID-19) nucleocapsid antibody as detection antibody at concentration of 1 μg/mL. HRP-conjuagted mouse IgG antibody was diluted at 1/10000 dilution and used to detect the primary antibody.
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![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.com/ps/products/281/ab281301/Images/ab281301-1-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-sandwichelisa.jpg)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 µg/mL and a HRP-conjugated ab281299 as detection antibody at concentration of 1 µg/mL.
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![Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.com/ps/products/281/ab281301/Images/ab281301-3-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-indirectelisa.jpg)
Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (1000-15.63 ng/mL). Coated protein probed with ab281301 at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
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![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.com/ps/products/281/ab281301/Images/ab281301-6-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-westernblot-293tcells.jpg)
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.com/ps/products/281/ab281301/Images/ab281301-2-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-westernblot-veroe6cells.jpg)
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301) at 1/5000 dilution
Lane 1 : Vero E6 whole cell extracts
Lane 2 : SARS-CoV-2 nucleocapsid transfected Vero E6 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.com/ps/products/281/ab281301/Images/ab281301-457463-SARSCoV2-nucleocapsid-protein-antibody-HL5410-elisa.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 μg/mL and a HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody as detection antibody at concentration of 1 μg/mL.
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![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.com/ps/products/281/ab281301/Images/ab281301-457464-SARSCoV2-nucleocapsid-protein-antibody-HL5410-elisa2.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 μg/mL and a HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody as detection antibody at concentration of 1 μg/mL.