Anti-PTPRH antibody [EPR20897-15] - BSA and Azide free (ab275934)
![Anti-PTPRH antibody [EPR20897-15] - BSA and Azide free (ab275934)](https://www.abcam.com/ps/products/275/ab275934/Images/ab275934-412161-anti-ptprh-antibody-epr2089715-western-blot-adenocarcinoma.jpg "Anti-PTPRH antibody [EPR20897-15] - BSA and Azide free (ab275934)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20897-15] to PTPRH - BSA and Azide free
- Suitable for: WB, ICC
- Reacts with: Human
Overview
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Product name
Anti-PTPRH antibody [EPR20897-15] - BSA and Azide free
See all PTPRH primary antibodies -
Description
Rabbit monoclonal [EPR20897-15] to PTPRH - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HT-29 and A431 whole cell lysates. ICC: HT-29 cells.
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General notes
ab275934 is the carrier-free version of ab231767. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275934 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20897-15 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-SAP1 antibody [EPR20897-15] - BSA and Azide free (ab275934)All lanes : Anti-PTPRH antibody [EPR20897-15] (ab231767) at 1/1000 dilution
Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 122 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?This data was developed using ab231767, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: SW480 and Low expression: HCT 116 (PMID: 28713969).
Exposure time: 37 seconds.
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![Immunocytochemistry - Anti-SAP1 antibody [EPR20897-15] - BSA and Azide free (ab275934)](https://www.abcam.com/ps/products/275/ab275934/Images/ab275934-1-anti-ptprh-antibody-epr2089715-immunocytochemistry-ht29.jpg)
Immunocytochemistry - Anti-SAP1 antibody [EPR20897-15] - BSA and Azide free (ab275934)This data was developed using ab231767, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HT-29 cells labelling PTPRH with ab231767 at 1/50 (11.42 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HT-29 cell line. Negative control: SW480 PMID: 28713969). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Western blot - Anti-SAP1 antibody [EPR20897-15] - BSA and Azide free (ab275934)](https://www.abcam.com/ps/products/275/ab275934/Images/ab275934-412162-anti-ptprh-antibody-epr2089715-western-blot-adenocarcinoma1.jpg)
Western blot - Anti-SAP1 antibody [EPR20897-15] - BSA and Azide free (ab275934)All lanes : Anti-PTPRH antibody [EPR20897-15] (ab231767) at 1/1000 dilution
Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HT-29 whole cell lysate treated with PNGase F
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 122 kDa
Observed band size: 122,250 kDa why is the actual band size different from the predicted?This data was developed using ab231767, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 59 seconds.
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![Anti-PTPRH antibody [EPR20897-15] - BSA and Azide free (ab275934)](https://www.abcam.com/ps/products/275/ab275934/Images/ab275934-4-benefits-of-recombinant-antibodies.png)
Anti-PTPRH antibody [EPR20897-15] - BSA and Azide free (ab275934)