Anti-p75 NGF Receptor antibody [MC-192] (ab6172)
![Anti-p75 NGF Receptor antibody [MC-192] (ab6172)](https://www.abcam.com/ps/products/6/ab6172/Images/ab6172-12138-anti-p75-ngf-receptor-antibody-mc-192-immunofluorescence.jpg "Anti-p75 NGF Receptor antibody [MC-192] (ab6172)")
Key features and details
- Mouse monoclonal [MC-192] to p75 NGF Receptor
- Suitable for: ICC
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-p75 NGF Receptor antibody [MC-192]
See all p75 NGF Receptor primary antibodies -
Description
Mouse monoclonal [MC-192] to p75 NGF Receptor -
Host species
Mouse -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Rat PC-12 cells.
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Positive control
- This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.
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General notes
A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor. -
Clonality
Monoclonal -
Clone number
MC-192 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-p75 NGF Receptor antibody [MC-192] (ab6172)
ab6172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6172 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
