Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

Key features and details

  • Mouse monoclonal [1A9] to SARS spike glycoprotein
  • Suitable for: ICC/IF, WB, Flow Cyt, IP, Sandwich ELISA
  • Isotype: IgG1

Overview

  • Product name

    Anti-SARS spike glycoprotein antibody [1A9]
    See all SARS spike glycoprotein primary antibodies

  • Description

    Mouse monoclonal [1A9] to SARS spike glycoprotein

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt, IP, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV, SARS-CoV-2

  • Immunogen

    Tissue, cells or virus corresponding to Human coronavirus SARS spike glycoprotein aa 1029-1192.

  • Positive control

    • WB: Infected Caco-2 cells; SARS-CoV-2 transfected HEK-293T cell lysate; SARS-Cov1 spike protein transfected Expi cell lysate, SARS-Cov1 3xFlag spike protein transfected Expi cell lysate, SARS-Cov2 spike protein transfected Expi cell lysate and SARS-Cov2 3xFlag spike protein transfected Expi cell lysate. ICC/IF: SARS-CoV-2 transfected BHK-21, Vero E6, COS-7 cells. IP: SARS-CoV-2 transfected HEK-293T cells. Flow Cyt: Infected HEK-293T cells.

  • General notes

    Applications overview

    Tick: Tested and Guaranteed to work      X: Will not work      —: No data

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    All lanes : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1 µg/ml

    Lane 1 : Mock transfected human Expi cell lysate
    Lane 2 : PDL-1 Flag transfected human Expi cell lysate
    Lane 3 : SARS-Cov1 spike protein transfected human Expi cell lysate
    Lane 4 : SARS-Cov1 3xFlag spike protein transfected human Expi cell lysate
    Lane 5 : SARS-Cov2 spike protein transfected human Expi cell lysate
    Lane 6 : SARS-Cov2 3xFlag spike protein transfected human Expi cell lysate
    Lane 7 : MERS Spike protein transfected human Expi cell lysate
    Lane 8 : MERS 3xFlag Spike protein transfected human Expi cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 139 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 8: Merged signal (red and green). Green - ab273433 observed at 200 kDa. Red - loading control.

    ab273433 was shown to react with SARS spike glycoprotein in western blot. Membranes were blocked in 3% milk before incubation with ab273433 overnight at 4°C at 1 ug/ml. Blots were incubated with Donkey anti-Mouse IgG H&L(IRDye® 800CW) preabsorbed (ab216774) antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Mock and SARS-CoV-2-transfected COS-7 cells stained for SARS-CoV-2 (COVID-19) spike (green) using ab273433 at 1/2000 dilution in ICC/IF. Nuclei were counterstained with DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Methanol-fixed Vero E6 cells infected with SARS-CoV-2 (MOI of 1) stained for SARS-CoV-2 (green) using ab273433 at 1/2000 dilution in ICC/IF. Counterstained with DAPI (Blue).

  • Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Flow cytometric analysis of HEK-293T cells infected with recombinant vaccinia virus carrying the spike gene, using ab273433.

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    All lanes : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T whole cell extract
    Lane 2 : Transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG antibody

    Predicted band size: 139 kDa

  • Sandwich ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Sandwich ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Sandwich ELISA detection of SARS spike glycoprotein using ab273433 as the capture antibody at 5 µg/mL. An alternative antibody was used as the detection antibody at 1 µg/mL followed by a Rabbit IgG antibody (HRP) at 1/10000 to detect the primary antibody.

  • Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    SARS-CoV-2 Spike was immunoprecipitated from 2μg of HEK-293T whole cell lysate with ab273433. Western blot was performed from the immunoprecipitate using ab273433. EasyBlot HRP-conjugated anti mouse IgG antibody.


    Lane 1: HEK-293T lysate 2 μg (Input).
    Lane 2: EasyBlot HRP-conjugated anti mouse IgG antibody.
    Lane 3: ab273433 IP in HEK-293T lysate.

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    4% paraformaldehyde-fixed BHK-21 cells stained for SARS-CoV-2 (COVID-19) spike (green) using ab273433 at 1/2000 dilution in ICC/IF.

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Lane 1 : Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Lanes 2-3 : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1/1000 dilution

    Lane 1 : Mock
    Lane 2 : SARS-CoV infected Caco-2 whole cell extract
    Lane 3 : SARS-CoV-2 infected Caco-2 whole cell extract

    Predicted band size: 139 kDa

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