Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Adiponectin antibody [EPR17019] (ab181281)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17019] to Adiponectin
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Adiponectin antibody [EPR17019]
    See all Adiponectin primary antibodies

  • Description

    Rabbit monoclonal [EPR17019] to Adiponectin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse plasma, serum, placenta, white adipose, kidney and liver lysates. IHC-P: Mouse and rat adipose tissues. ICC/IF: 3T3-L1 cells. Flow Cyt: 3T3-L1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Adiponectin antibody [EPR17019] (ab181281)
    Western blot - Anti-Adiponectin antibody [EPR17019] (ab181281)
    All lanes : Anti-Adiponectin antibody [EPR17019] (ab181281) at 1/1000 dilution

    Lane 1 : Mouse plasma lysate
    Lane 2 : Mouse serum lysate
    Lane 3 : Mouse placenta lysate
    Lane 4 : Mouse white adipose lysate
    Lane 5 : Mouse kidney lysate
    Lane 6 : Mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



     

    Exposure times: Lanes 1-2,4-5: 1 second; Lane 3: 3 minutes; Lane 6: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Adiponectin antibody [EPR17019] (ab181281)
    Immunocytochemistry/ Immunofluorescence - Anti-Adiponectin antibody [EPR17019] (ab181281)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 3T3-L1 (mouse embryonic fibroblast cell line) cells labeling Adiponectin with ab181281 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on 3T3-L1 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)

    Immunohistochemical analysis of paraffin-embedded mouse adipose tissue labeling Adiponectin with ab181281 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse adipocytes (PMID: 25676879). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Adiponectin antibody [EPR17019] (ab181281)
    Flow Cytometry - Anti-Adiponectin antibody [EPR17019] (ab181281)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized 3T3-L1 (mouse embryonic fibroblast cell line) cell line labeling Adiponectin with ab181281 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)

    Immunohistochemical analysis of paraffin-embedded rat adipose tissue labeling Adiponectin with ab181281 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat adipocytes (PMID: 25676879). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Adiponectin antibody [EPR17019] (ab181281)
    Anti-Adiponectin antibody [EPR17019] (ab181281)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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