Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADIPOR1 antibody [EPR6626] (ab126611)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6626] to ADIPOR1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ADIPOR1 antibody [EPR6626]
    See all ADIPOR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6626] to ADIPOR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ADIPOR1 aa 1-100 (intracellular). The exact sequence is proprietary.

  • Positive control

    • WB: Rat and mouse heart tissue lysates and human skeletal muscle tissue lysate. IHC-P: Human kidney tissue. ICC/IF: HeLa cells.

  • General notes

     This product was previously labelled as Adiponectin Receptor 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    Western blot - Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    All lanes : Purified ab126611 at 1/1000 dilution

    Lane 1 : Mouse heart, 20 µg
    Lane 2 : Rat heart, 20 µg

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    5% NFDM/TBST dilution buffer

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] (ab126611)

    Immunohistochemical staining of paraffin embedded human endometrium with purified ab126611 at a dilution of 1/200. The seconday was a HRP polymer for rabbit IgG amd the counter stain used was hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] (ab126611)

    Immunocytochemistry of HeLa cells fixed in 4% PFA with purified ab126611 at a dilution of 1/400. An Alexa Fluor® goat anti-rabbit antibody was used as the secondary and the sample was counter stained wtih DAPI.

  • Flow Cytometry - Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    Flow Cytometry - Anti-ADIPOR1 antibody [EPR6626] (ab126611)

    Overlay histogram showing HepG2 cells stained with unpurified ab126611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126611, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] (ab126611)

    Immunohistochemical staining of ADIPOR1 in formalin-fixed, paraffin-embedded human kidney tissue by unpurified ab126611, at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    Western blot - Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    Anti-ADIPOR1 antibody [EPR6626] (ab126611) at 1/1000 dilution (Purified) + Human skeletal muscle at 10 µg

    Secondary
    HRP anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • OI-RD Scanning - Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    OI-RD Scanning - Anti-ADIPOR1 antibody [EPR6626] (ab126611)

    Equilibrium disassociation constant (KD) with unpurified ab126611.
    Learn more about KD

    Click here to learn more about KD

  • Anti-ADIPOR1 antibody [EPR6626] (ab126611)
    Anti-ADIPOR1 antibody [EPR6626] (ab126611)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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