Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11227] to MHC Class II beta - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free
    See all MHC Class II beta primary antibodies

  • Description

    Rabbit monoclonal [EPR11227] to MHC Class II beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji cell lysate, human tonsil. ICC/IF: Raji cells. IHC-P: human tonsil tissue, human endometrium tissue.

  • General notes

    ab249550 is the carrier-free version of ab170867.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11227

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)
    Immunocytochemistry - Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)
    This data was developed using ab170867, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Raji cells labeling MHC Class II beta with ab170867 at 1/100 dilution (green). DAPI nuclear staining (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)
    This data was developed using ab170867, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human uterus tissue labeling MHC Class II beta with ab170867 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)
    This data was developed using ab170867, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Formalin-fixed, paraffin-embedded human lymphoid follicle labeling MHC Class II beta with ab170867 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)
    Western blot - Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)
    All lanes : Anti-MHC Class II beta antibody [EPR11227] (ab170867) at 1/1000 dilution

    Lane 1 : Human tonsil lysates
    Lane 2 : Human thymus lysates
    Lane 3 : Raji lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 29 kDa



    This data was developed using ab170867, the same antibody clone in a different buffer formulation.

     

  • Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)
    Anti-MHC Class II beta antibody [EPR11227] - BSA and Azide free (ab249550)

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