Withaferin A from Withania somnifera (ab120644)
Key features and details
- Naturally occurring withanolide/steroidal lactone with anticancer properties
- CAS Number: 5119-48-2
- Purity: > 98%
Soluble in ethanol
- Form / State: Solid
- Source: Withania somnifera
Overview
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Product name
Withaferin A from Withania somnifera -
Description
Naturally occurring withanolide/steroidal lactone with anticancer properties -
Alternative names
- Withaferine A
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Biological description
Naturally occurring withanolide/steroidal lactone with anticancer properties. Demonstrates a number of cellular effects including the covalent binding to annexin II, inhibition of chymotrypsin-like activity, PKC and caspase 3. Causes translocation of cyctochrome C to the nucleous and the cleavage of PLC-γ1. Down-regulates Notch 1, 3, cdc25C, phosphorylated Akt and bcl-2 proteins.
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Purity
> 98% -
CAS Number
5119-48-2 -
Chemical structure
Properties
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Chemical name
(4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
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Molecular weight
470.60 -
Molecular formula
C28H38O6 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Withania somnifera
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Research areas
Images
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Functional Studies - Withaferin A from Withania somnifera (ab120644)ab7973 staining Bcl2 in HEK 293 cells treated with withaferin A from Withania somnifera (ab120644), by ICC/IF. Decrease in Bcl2 expression with increased concentration of withaferin A from Withania somnifera, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120644 (withaferin A from Withania somnifera) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7973 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.