Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 123 964 ₸

Order now and get it on Tuesday March 02, 2021

Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20991] to eNOS (phospho S1177)
Suitable for: IP, WB, Dot blot
Reacts with: Mouse, Human

Product name

Anti-eNOS (phospho S1177) antibody [EPR20991]
See all eNOS primary antibodies
Description

Rabbit monoclonal [EPR20991] to eNOS (phospho S1177)
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HUVEC (+/- VEGF or pervanadate) whole cell lysates; bEND.3 whole cell lysate. IP: HUVEC treated with VEGF whole cell lysate. Dot blot: eNOS (phospho S1177) peptide.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20991
Isotype

IgG
Research areas

Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)

All lanes : Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) at 1/1000 dilution

Lane 1 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 2 : bEND.3 (mouse brain endothelioma cell line) treated with alkaline phosphatase for 1 hour, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 133 kDa
Observed band size: 140 kDa
why is the actual band size different from the predicted?

Exposure time: 2 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)

Dot blot analysis of eNOS (phospho S1177) labeled with ab215717 at 1/1000 dilution.

Lane 1: eNOS (phospho S1177) peptide.
Lane 2: eNOS non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Exposure time: 1 minute.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)

eNOS (phospho S1177) was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) treated with 10 ng/ml VEGF for 5 min, whole cell lysate with ab215717 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215717 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: HUVEC treated with VEGF whole cell lysate 10 μg (Input).
Lane 2: ab215717 IP in HUVEC treated with VEGF whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215717 in HUVEC treated with VEGF whole cell lysate.

Exposure time: 30 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

The 60 kDa band may be a cleaved form of eNOS (PMID: 16195740).
Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)

All lanes : Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) at 1/1000 dilution

Lane 1 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell line) treated with 100 uM pervanadate for 10 min, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 133 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The 60 kDa band may be a cleaved form of eNOS (PMID: 16195740).
Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)

All lanes : Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) at 1/1000 dilution

Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell line) treated with 10 ng/ml VEGF for 5 min, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 133 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The 60 kDa band may be a cleaved form of eNOS (PMID: 16195740).
Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com