Anti-Rb (phospho S788) antibody [6HCLC] (ab277775)
![Anti-Rb (phospho S788) antibody [6HCLC] (ab277775)](https://www.abcam.com/ps/products/277/ab277775/Images/ab277775-2-antiPhosphoRbS788recombinantpolyclonalantibody6hclcWB.jpg "Anti-Rb (phospho S788) antibody [6HCLC] (ab277775)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [6HCLC] to Rb (phospho S788)
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Rb (phospho S788) antibody [6HCLC]
See all Rb primary antibodies -
Description
Rabbit recombinant multiclonal [6HCLC] to Rb (phospho S788) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide aa 784-791 (phospho S788).
Database link: P06400 -
Positive control
- WB: Colo 205 (treated with Nocadazole) whole cell extract, ICC: Colo 205 cells (treated with Nocodazole)
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
6HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rb antibody (ab277775)All lanes : Anti-Rb (phospho S788) antibody [6HCLC] (ab277775) at 2 µg/ml
Lane 1 : Colo 205 (human colon adenocarcinoma cell line) whole cell extracts at 30 µg
Lane 2 : Colo 205 (treated with 3 uM Nocodazole for 24 hours) whole cell extract at 30 µg
Lane 3 : Colo 205 whole cell extract (+ 10 uM phosphopeptide)
Lane 4 : Colo 205 (Nocozadole-treated) whole cell extract (+ 10 uM phosphopeptide) at 30 µg
Predicted band size: 106 kDa
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Immunocytochemistry - Anti-Rb antibody (ab277775)For immunofluorescence analysis COLO 205 cells were fixed and permeabilized for detection of endogenous RbpS788 using Anti-RbpS788 Recombinant Rabbit Multiclonal Antibody (ab277775, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI, and Alexa Fluor® 555 Rhodamine Phalloidin (1/300) was used for cytoskeletal F-actin (red) staining. Detection and localization of RbpS788 (green) in the nucleus can be clearly observed in cells treated with Nocodazole (3 uM, 16 h) as compared to untreated cells. Antibody specificity was demonstrated by competition with the RbpS788 peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.
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![Anti-Rb antibody [6HCLC] (ab277775)](https://www.abcam.com/ps/products/277/ab277775/Images/ab277775-3-benefits-of-recombinant-antibodies.jpg)
Anti-Rb antibody [6HCLC] (ab277775)