Human Urokinase type Plasminogen Activator Matched Antibody Pair Kit (ab221439)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 31.3 pg/ml - 2000 pg/ml
Overview
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Product name
Human Urokinase type Plasminogen Activator Matched Antibody Pair Kit
See all Urokinase kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
5.5 pg/ml -
Range
31.3 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Urokinase type Plasminogen Activator Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Urokinase type Plasminogen Activator.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab226904. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Urokinase type Plasminogen Activator Capture Antibody 2 x 50µg 1 x 50µg Human Urokinase type Plasminogen Activator Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Urokinase type Plasminogen Activator Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
Tissue specificity
Expressed in the prostate gland and prostate cancers. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ATF
- ATF uPA
- BDPLT5
see all -
Database links
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
Images
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Human urokinase type tissue plasminogen activator standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Urokinase type Plasminogen Activator Matched Antibody Pair Kit (ab221439)To learn more about the advantages of recombinant antibodies see here.
