PMSF, protease inhibitor (ab141032)
Key features and details
- Non-specific protease inhibitor
- CAS Number: 329-98-6
- Purity: > 99%
Chloroform
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
PMSF, protease inhibitor -
Description
Non-specific protease inhibitor -
Alternative names
- Phenylmethylsulfonyl fluoride
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Biological description
Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB. -
Purity
> 99% -
General notes
Unstable in water
Storage instructions: Hygroscopic, Under inert atmosphere.
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CAS Number
329-98-6 -
Chemical structure
Properties
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Chemical name
Phenylmethylsulfonyl fluoride
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Molecular weight
174.19 -
Molecular formula
C7H7FO2S -
PubChem identifier
4784 -
Storage instructions
Store at -20°C. Please see notes section. -
Solubility overview
Chloroform
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=S(F)(=O)Cc1ccccc1 -
Source
Synthetic
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Research areas
Images
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Cellular activation - PMSF, protease inhibitor (ab141032) Image from Song Y, et al. Plos One, 11(10), e0163165. Fig 5b,; doi: 10.1371/journal.pone.0163165
Analysis of protein expression level in the sclera. The scleral tissue was homogenized and solubilized in ice-cold PBS containing PMSF protease inhibitor. Lumican and collagen type I were both overexpressed at the protein level in the transgenic sclera. GAPDH was used as a control. Lane 1 to lane 5, transgenic mice. Lane 6 to lane 9, wild-type mice.
