Human Urokinase type Plasminogen Activator ELISA Kit (ab108917)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.25 ng/ml - 8 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Urokinase type Plasminogen Activator ELISA Kit
See all Urokinase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 7.2% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Tissue -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.2 ng/ml -
Range
0.25 ng/ml - 8 ng/ml -
Recovery
98.5 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Urokinase type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Urokinase type Plasminogen Activator (uPA) in plasma, serum, urine, milk, cell culture supernatants and tissue extracts.
A Urokinase type Plasminogen Activator specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Urokinase type Plasminogen Activator specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Urokinase type Plasminogen Activator captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 30X Biotinylated Human Urokinase type Plasminogen Activator Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Urokinase type Plasminogen Activator Microplate (12 x 8 well strips) 1 unit Urokinase type Plasminogen Activator Standard 1 vial -
Research areas
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Function
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
Tissue specificity
Expressed in the prostate gland and prostate cancers. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ATF
- ATF uPA
- BDPLT5
see all -
Database links
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108917.
