Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free
    See all Prostate Specific Antigen primary antibodies

  • Description

    Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human prostate cancer tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    ab271858 is the carrier-free version of ab76113. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)
    Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)
    All lanes : Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/1000 dilution (Purified)

    Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 15 µg
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 3 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 4 : DU 145 (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 29 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76113, the same antibody clone in a different buffer formulation.

    The expression profile observed is consistent with what has been described in the literature (PMID: 23405127).

  • Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)
    Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)
    All lanes : Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/1000 dilution

    Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
    Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) treated with 100nM DHT for 2 days whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 29 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?



    This data was developed using ab76113, the same antibody clone in a different buffer formulation.

    The expression profile observed is consistent with what has been described in the literature (PMID: 23405127).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)

    This data was developed using ab76113, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate cancer tissue sections labeling Prostate Specific Antigen with purified ab76113 at 1/1000 dilution (0.51 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • OI-RD Scanning - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)
    OI-RD Scanning - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76113).

  • Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)
    Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (ab271858)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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