Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427)
![Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427)](https://www.abcam.com/ps/products/238/ab238427/Images/ab238427-451828-anti-prc1-antibody-ep1513y-westernblot-human-mouse-rat.jpg "Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1513Y] to PRC1 - BSA and Azide free
- Suitable for: ICC, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PRC1 antibody [EP1513Y] - BSA and Azide free
See all PRC1 primary antibodies -
Description
Rabbit monoclonal [EP1513Y] to PRC1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: HeLa cells Flow cyt: HeLa cells WB: HeLa, C6 and C2C12 whole cell lysates. Mouse and rat kidney tissue.
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General notes
Ab238427 is the carrier-free version of ab51248. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238427 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1513Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427)All lanes : Anti-PRC1 antibody [EP1513Y] (ab51248) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDaab51248 recognise two isoforms of PRC1. But we are unsure how to define the extra bands at ~50kDa.
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![Flow Cytometry - Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427)](https://www.abcam.com/ps/products/238/ab238427/Images/ab238427-6-anti-prc1-antibody-ep1513y-flowcytometry-hela-human.jpg)
Flow Cytometry - Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427)This data was developed using 238427, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PRC1 with Purified 238427 at 1:30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). -
![Immunocytochemistry - Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427)](https://www.abcam.com/ps/products/238/ab238427/Images/ab238427-5-anti-prc1-antibody-ep1513y-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427)This data was developed using 238427, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PRC1 with Purified 238427 at 1:50 dilution (5.06 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Immunocytochemistry - Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427)](https://www.abcam.com/ps/products/238/ab238427/Images/ab238427-330178-anti-prc1-antibody-ep1513y-immunofluorescence.jpg)
Immunocytochemistry - Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (ab238427) This image is courtesy of an anonymous Abreview.ab51248 staining PRC1 in human breast cancer cells by ICC/IF. The cells were paraformaldehyde fixed and blocked in 1% serum for 1 hour at 37°C without permeation step. The primary antibody was diluted 1/100 (PBS) and incubated with sample for 1 hour at 20°C. An Alexa Fluor® 488 conjugated donkey polyclonal to rabbit IgG, diluted 1/200 was used as secondary.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51248).
