Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Prostate Specific Antigen antibody [EP1588Y]
    See all Prostate Specific Antigen primary antibodies

  • Description

    Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen

  • Host species

    Rabbit

  • Specificity

    The antibody provided good results in western blot when tested in human prostate lysate. Unfortunately, when 22RV1 cell lysate was used no band was detected and inconsistent results were obtained with LNCaP cell lysate (where different lots of cell lysate were used for the test). Please contact our Scientific Support if you have any feedback and/or questions.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Prostate Specific Antigen aa 200-300 (C terminal). The exact sequence is proprietary.
    Database link: P07288

  • Positive control

    • WB: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate and LNCaP (Human prostate carcinoma epithelial cell) treated with 100nM DHT for 2 days whole cell lysate IHC-P: Human prostate cancer tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
    Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
    Lanes 1-2 : Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/1000 dilution (Purified)
    Lane 3 : Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/1000 dilution

    Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 29 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?



    The expression profile observed is consistent with what has been described in the literatures (PMID: 24906821, 17620434 and 25076860).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate cancer tissue sections labeling Prostate Specific Antigen with purified ab76113 at 1/1000 dilution (0.51 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
    Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
    All lanes : Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/1000 dilution

    Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
    Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) treated with 100nM DHT for 2 days whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 29 kDa

  • OI-RD Scanning - Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
    OI-RD Scanning - Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
    Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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