Human CD177 ELISA Kit (PRV1) (ab272195)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12.83 pg/ml
- Range: 156.3 pg/ml - 10000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD177 ELISA Kit (PRV1)
See all CD177 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.3% Inter-assay Sample n Mean SD CV% Serum 3 6% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12.83 pg/ml -
Range
156.3 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 91 86% - 97% Hep Plasma 88 83% - 93% EDTA Plasma 91 83% - 97% Cit plasma 98 95% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD177 ELISA kit (ab272195) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD177 protein in serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD177 with 12.83 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD177 (PRV1) is a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation by physical association with β2 integrins, particularly CD11b/CD18, and in addition to forming complexes with neutrophil proteinase 3 for degranulation and superoxide production. CD177 can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mouse, rat, cow share 57%, 49%, and 52% sequence homology with human CD177, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD177 (PRV1) Capture Antibody 1 x 600µl 10X Human CD177 (PRV1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CD177 (PRV1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Tissue specificity
Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). -
Sequence similarities
Contains 2 UPAR/Ly6 domains. -
Post-translational
modificationsN-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CD177
- CD177 antigen
- CD177 molecule
see all -
Database links
- Entrez Gene: 57126 Human
- Omim: 162860 Human
- SwissProt: Q8N6Q3 Human
- Unigene: 232165 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD177 standard curve in Sample Diluent NS.The CD177 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD177 in human serum and plasma samples.The concentrations of CD177 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 11,306 pg/mL in serum, 9,280 pg/mL in plasma (citrate), 9,383 pg/mL in plasma (EDTA), and 12,750 pg/mL in plasma (heparin).
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Sandwich ELISA - Human CD177 ELISA Kit (PRV1) (ab272195)To learn more about the advantages of recombinant antibodies see here.
