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Epigenetics and Nuclear Signaling Chromatin Remodeling Swi / Snf

Anti-SNF2L antibody [2H7B9] (ab233644)

Anti-SNF2L antibody [2H7B9] (ab233644)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [2H7B9] to SNF2L
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-SNF2L antibody [2H7B9]
    See all SNF2L primary antibodies
  • Description

    Mouse monoclonal [2H7B9] to SNF2L
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human SNF2L aa 933-1070. The exact sequence is proprietary. (Expressed in E.coli).
    Database link: P28370

  • Positive control

    • WB: PANC-1, HEK-293, SW620, HT-29, SH-SY5Y ans SK-OV-3 cell lysates; SNF2L (aa 933-1070)-hIgGFc transfected HEK-293 cell lysate; Human SNF2L (aa 933-1070) recombinant protein. Flow Cyt: NIH/3T3 cells. IHC-P: Human esophageal cancer and stomach cancer tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    2H7B9
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Images

  • Western blot - Anti-SNF2L antibody [2H7B9] (ab233644)
    Western blot - Anti-SNF2L antibody [2H7B9] (ab233644)
    Anti-SNF2L antibody [2H7B9] (ab233644) at 1/500 dilution + Human SNF2L (aa 933-1070) recombinant protein

    Predicted band size: 123 kDa



    Expected MW is 42.4 kDa.

  • Western blot - Anti-SNF2L antibody [2H7B9] (ab233644)
    Western blot - Anti-SNF2L antibody [2H7B9] (ab233644)
    All lanes : Anti-SNF2L antibody [2H7B9] (ab233644) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : SNF2L (aa 933-1070)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 123 kDa

  • Western blot - Anti-SNF2L antibody [2H7B9] (ab233644)
    Western blot - Anti-SNF2L antibody [2H7B9] (ab233644)
    All lanes : Anti-SNF2L antibody [2H7B9] (ab233644) at 1/500 dilution

    Lane 1 : PANC-1 (human pancreatic epithelial cancinoma cell line) cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 3 : SW620 cell lysate
    Lane 4 : Ht-29 (human colorectal adenocarcinoma cell line) cell lysate
    Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
    Lane 6 : SK-OV-3 (human ovarian cancer cell line) cell lysate

    Predicted band size: 123 kDa

  • Flow Cytometry - Anti-SNF2L antibody [2H7B9] (ab233644)
    Flow Cytometry - Anti-SNF2L antibody [2H7B9] (ab233644)

    Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cell line labeling SNF2L with ab233644 at 1/200 dilution (green) compared with a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [2H7B9] (ab233644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [2H7B9] (ab233644)

    Paraffin-embedded human esophageal cancer tissue stained for SNF2L using ab233644 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [2H7B9] (ab233644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [2H7B9] (ab233644)

    Paraffin-embedded human stomach cancer tissue stained for SNF2L using ab233644 at 1/200 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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