Anti-SNF2L antibody [2H7B9] (ab233644)
Key features and details
- Mouse monoclonal [2H7B9] to SNF2L
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-SNF2L antibody [2H7B9]
See all SNF2L primary antibodies -
Description
Mouse monoclonal [2H7B9] to SNF2L -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIHC-P HumanWB Human -
Immunogen
Recombinant fragment within Human SNF2L aa 933-1070. The exact sequence is proprietary. (Expressed in E.coli).
Database link: P28370 -
Positive control
- WB: PANC-1, HEK-293, SW620, HT-29, SH-SY5Y ans SK-OV-3 cell lysates; SNF2L (aa 933-1070)-hIgGFc transfected HEK-293 cell lysate; Human SNF2L (aa 933-1070) recombinant protein. Flow Cyt: NIH/3T3 cells. IHC-P: Human esophageal cancer and stomach cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2H7B9 -
Isotype
IgG1 -
Research areas
Images
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Anti-SNF2L antibody [2H7B9] (ab233644) at 1/500 dilution + Human SNF2L (aa 933-1070) recombinant protein
Predicted band size: 123 kDaExpected MW is 42.4 kDa.
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All lanes : Anti-SNF2L antibody [2H7B9] (ab233644) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : SNF2L (aa 933-1070)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 123 kDa
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All lanes : Anti-SNF2L antibody [2H7B9] (ab233644) at 1/500 dilution
Lane 1 : PANC-1 (human pancreatic epithelial cancinoma cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : SW620 cell lysate
Lane 4 : Ht-29 (human colorectal adenocarcinoma cell line) cell lysate
Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 6 : SK-OV-3 (human ovarian cancer cell line) cell lysate
Predicted band size: 123 kDa
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Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cell line labeling SNF2L with ab233644 at 1/200 dilution (green) compared with a negative control (red).
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Paraffin-embedded human esophageal cancer tissue stained for SNF2L using ab233644 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human stomach cancer tissue stained for SNF2L using ab233644 at 1/200 dilution in immunohistochemical analysis.