Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)
![Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)](https://www.abcam.com/ps/products/192/ab192833/Images/ab192833-254790-192833WBa.jpg "Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19282] to Pellino 1 + Pellino 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-Pellino 1 + Pellino 2 antibody [EPR19282]
See all Pellino 1+Pellino 2 primary antibodies -
Description
Rabbit monoclonal [EPR19282] to Pellino 1 + Pellino 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHumanRecombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human Pellino 1 and Pellino 2 full length recombinant proteins; Ramos, PC-12, Jurkat, Daudi, HeLa, C6 and NIH/3T3 whole cell lysates; RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate; Mouse and Rat brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19282 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/5000 dilution
Lane 1 : Human Pellino 1 full length recombinant protein
Lane 2 : Human Pellino 2 full length recombinant protein
Lane 3 : Human Pellino 3 full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDaBlocking/dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1:1 second; Lane 2 and 3:1 minute.
Human Pellino 1 full length recombinant protein contains aa1-418 with a His-Tag®. Human Pellino 2 full length recombinant protein contains aa1-420 with a His-Tag®. Human Pellino 3 full length recombinant protein contains aa1-469 with a His-Tag®. All three recombinant proteins were made in-house.
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![Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)](https://www.abcam.com/ps/products/192/ab192833/Images/ab192833-254789-192833WBb.jpg)
Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 1 µg/ml LPS for 6 hours, whole cell lysate
Lane 4 : Untreated PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) treated with 1 µg/ml LPS for 6 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
The expression of Pellino 1 can be induced by LPS. (PMID: 21204785).
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![Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)](https://www.abcam.com/ps/products/192/ab192833/Images/ab192833-254788-192833WBc.jpg)
Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)](https://www.abcam.com/ps/products/192/ab192833/Images/ab192833-254787-192833WBd.jpg)
Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
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![Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)](https://www.abcam.com/ps/products/192/ab192833/Images/ab192833-5-benefits-of-recombinant-antibodies.png)
Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)
