Mouse MAPK 12 Matched Antibody Pair Kit (ab218177)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
-
Product name
Mouse MAPK 12 Matched Antibody Pair Kit
See all p38 gamma/MAPK12 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
26.5 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MAPK 12 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MAPK 12.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208347. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse MAPK 12 Capture Antibody 2 x 50µg 1 x 50µg Mouse MAPK 12 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse MAPK 12 Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. -
Tissue specificity
Highly expressed in skeletal muscle and heart. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. -
Cellular localization
Cytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5. - Information by UniProt
-
Alternative names
- ERK 6
- ERK-6
- ERK6
see all -
Database links
- Entrez Gene: 29857 Mouse
- SwissProt: O08911 Mouse
- Unigene: 38343 Mouse
Images
-
Mouse SAPK3 standard curve.
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Mouse MAPK 12 Matched Antibody Pair Kit (ab218177)To learn more about the advantages of recombinant antibodies see here.
