Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Drosophila melanogaster

Overview

  • Product name

    Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free
    See all AMPK alpha 1+AMPK alpha 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Drosophila melanogaster

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Molt-4 cell lysate. HEK-293 cell lysate. Fruit fly lysate. Rat brain lysate, Mouse heart lysate.

  • General notes

    ab271892 is the carrier-free version of ab133448. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (unpurified)

    Lane 1 : 293 cell lysate
    Lane 2 : Lysate of 293 cells treated with lambda phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)

    Lane 1 : Untreated HEK-293 cell lysate
    Lane 2 : HEK-293 cell lysate treated with Lambda phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)


    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Molt-4 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1000 cells (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)


    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)


    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Fruit fly lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)


    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/2000 dilution

    Lane 1 : HEK-293 (human embryonic kidney) whole cell lysates
    Lane 2 : HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 phospho pT183 and AMPK alpha 2 phospho pT172 peptides
    Lane 3 : HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 and AMPK alpha 2 non-phospho peptides

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), HRP conjugated (ab97051) at 1/20000 dilution


    Blocking and dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

  • OI-RD Scanning - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    OI-RD Scanning - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133448).

  • Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
    Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)

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For licensing inquiries, please contact partnerships@abcam.com

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