Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] (ab133447)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR826(2)] to Amyloid Precursor Protein (phospho T743)
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)]
See all Amyloid Precursor Protein primary antibodies -
Description
Rabbit monoclonal [EPR826(2)] to Amyloid Precursor Protein (phospho T743) -
Host species
Rabbit -
Specificity
This antibody only detects Amyloid beta precursor protein phosphorylated at Threonine 743. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Amyloid Precursor Protein aa 700 to the C-terminus (phospho T743). The exact sequence is proprietary.
-
Positive control
- WB: Human brain lysate
-
General notes
The immunogen used for this product is within Human Amyloid Precursor Protein aa 750 to the C-terminus and therefore may detect gamma secretase fragments 50, 57 and 59 in addition to fragments C31, C80, C83 and C99. Cross-reactivity with these fragments has not been confirmed experimentally.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR826(2) -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] (ab133447) at 1/1000 dilution
Lane 1 : Human brain lysate
Lane 2 : Human brain lysate treated with lambda phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 86 kDa
-