Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)
![Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)](https://www.abcam.com/ps/products/250/ab250695/Images/ab250695-406053-anti-aconitase-1-aco1-antibody-epr7226-2-bsa-and-azide-free-western-blot-knockout-human-lysates.jpg "Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7226(2)] to Aconitase 1/ACO1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free
See all Aconitase 1/ACO1 primary antibodies -
Description
Rabbit monoclonal [EPR7226(2)] to Aconitase 1/ACO1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250695 is the carrier-free version of ab183721 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250695 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Aconitase 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7226(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1 µg/ml
Lane 1 : Wild-type U20S whole cell lysate
Lane 2 : ACO1 knockout U20S whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 98 kDaThis data was developed using ab183721, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - ab183721 observed at 98 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab183721 was shown to specifically react with ACO1 in wild-type U20S cells as signal was lost in ACO1 knockout cells. Wild-type and ACO1 knockout samples were subjected to SDS-PAGE. ab183721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)](https://www.abcam.com/ps/products/250/ab250695/Images/ab250695-403103-anti-aconitase-1-aco1-antibody-epr7226-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 98 kDaThis data was developed using ab183721, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)](https://www.abcam.com/ps/products/250/ab250695/Images/ab250695-398155-anti-aconitase-1-aco1-antibody-epr7226-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : T.T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 98 kDaThis data was developed using ab183721, the same antibody clone in a different buffer formulation.
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![Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)](https://www.abcam.com/ps/products/250/ab250695/Images/ab250695-4-benefits-of-recombinant-antibodies.png)
Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (ab250695)